Abstract: BACKGROUND ＆ AIM:Centromeric dots(Cd)variation of HepG2 cells and its relation with aneuploidy aberration were studied. MATERIAL AND METHODS: A Cd-NOR in-phase argentums dye analysis technique modified by this laboratory was used to study centromeric dots(Cd)variation of HepG2 cells. RESULTS： Frequency of Cd loss in HepG2 cells was 2.30 ％, Frequency of Cd duplication laggard was 1.02 ％, Frequency of little Cd was 2.58 ％, Frequency of Cd-NOR amalgamation was 0.64 ％. Frequency of Cd loss, frequency of Cd duplication laggard and frequency of little Cd in HepG2 cells were significantly higher than that of normal embryonic villi cells(P<0.05). Frequency of Cd-NOR amalgamation showed no difference between HepG2 cells and normal embryonic villi. CONCLUSION：Cd loss,Cd duplication laggard and little Cd might be related with aneuploidy aberration of HepG2 cells.

Key words: HepG2 cell, Centromeric dots, Aneuploidy aberration